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. 2001 Dec 11;98(26):15107–15112. doi: 10.1073/pnas.251234498

Figure 3.

Figure 3

Analysis of cellular hMLH1 mRNA content. Multiplexed RT-PCR was performed with primers specific for the hMLH1 and hprt genes. LoVo and DLD-1 cell lines, as well as SW48 cells treated for 48 h with various doses of 5-aza-2′-deoxycytidine (2 μM and 10 μM Aza), were used as positive controls for hMLH1 expression. Four hprt− single cell clones obtained from MSH6 (+/+) or (−/−) cell lines were analyzed for hMLH1 expression. Single-cell clones 1 and 2 are mutants with frameshift hprt mutations and single-cell clones 3 and 4 are mutants with base-substitution hprt mutations.