FIGURE 1.
Effects of GF2F diet. (A) Schematic overview of the experimental design. Effects of GF2F diet on influenza vaccine–specific (B) DTH response, (C) ear weight increase, and (D) IgG1 and (E) IgG2a concentrations in serum of vaccinated mice at day 31. DTH response and ear weight were measured 24 h after ear challenge. IgG1 and IgG2a levels in serum were measured by means of ELISA. The Mann-Whitney U-test was used. Data are presented as mean ± SEM for n = 8–9/group in panels B–E. Significantly different from CON: *P < 0.05; **P < 0.01. AU, arbitrary unit; CON, control; DTH, delayed type hypersensitivity; GF2F, scGOS/lcFOS/2′FL (2′FL, 2′-fucosyllactose; FOS, fructo-oligosaccharides; GOS, galacto-oligosaccharides; lc, long chain; sc, short chain).