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. 2019 Apr 24;149(5):856–869. doi: 10.1093/jn/nxz006

FIGURE 2.

FIGURE 2

Effects of GF2F diet on the percentage of (B) CD11c+ MHCII+ DCs; (C) CD103+ DCs of CD11c+ MHCII+ cells; (D) maturation status of CD013+ DCs by CD86+ and CD40+ MFI; (F) the percentage of CD27+ B cells of total CD19+ B220+ B cells; (H) percentage of CD4+ cells in the total lymphocytes; (I) percentage of CD25+ Foxp3+ Treg; and (J) CD69+ Tbet+ Th1 of CD4+ T cells in the MLNs of vaccinated mice. Gating and representative plots of (A) CD103+ DCs (CD11c+ MHCII+); (E) CD27+ B cells; (G) Foxp3+ Treg and Tbet+ Th1 cells in the MLNs from CON and GF2F groups are shown. The Mann-Whitney U test was used. Data are presented as mean ± SEM for n = 8–9/group. Significantly different from CON: *P < 0.05; **P < 0.01. CON, control; DC, dendritic cell; GF2F, scGOS/lcFOS/2′FL (2′FL, 2′-fucosyllactose; FOS, fructo-oligosaccharides; GOS, galacto-oligosaccharides; lc, long chain; sc, short chain); MFI, medium fluorescence intensity; MLN, mesenteric lymph node.