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. 2019 Apr 24;149(5):856–869. doi: 10.1093/jn/nxz006

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Copyright © American Society for Nutrition 2019.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of GF2F diet on (A) SCFA concentrations in cecal content (CC) and (B) feces of vaccinated mice, and (C) significant correlations with DTH responses. Feces were collected at day 30, and the cecum was collected at day 31 after killing the mice in order to measure the SCFAs. The absolute amounts of acetic acid, propionic acid, butyric acid, and SCFA mixture (acetic acid + propionic acid + butyric acid) are presented as mean ± SEM for n = 8–9/group. Statistical analysis with the Mann-Whitney U test was performed for panels A and B; *significantly different from CON, P < 0.05. Pearson's correlation analysis was performed for panel (C). CC, cecum content; DTH, delayed type hypersensitivity; GF2F, scGOS/lcFOS/2′FL (2′FL, 2′-fucosyllactose; FOS, fructo-oligosaccharides; GOS, galacto-oligosaccharides; lc, long chain; sc, short chain).