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. 2019 Apr 24;9:284. doi: 10.3389/fonc.2019.00284

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Copyright © 2019 Howard, Bearss, Subramaniyan, Tilley, Sridharan, Villa, Fraser and Raman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The LASP1-eIF4A interaction increases with CXCL12 stimulation in situ. The Proximity Ligation Assay (PLA) was used to visual the in situ interaction between LASP1 and eIF4A in 231S cells. Cells were stimulated with 20 nM CXCL12 for 5 min. 100 nM AMD3465 was added 30 min prior to stimulation. The single antibody control employs the PLA reaction using only the LASP1 antibody. Representative images of the PLA experiment are provided. Quantification indicates the average number of interactions/cell across multiple independent fields. (Single Ab Control: n = 39, -CXCL12: n = 46, +CXCL12: n = 29, +CXCL12/AMD3465: n = 15); Red-LASP1-eIF4A interaction, Green-phalloidin (actin), and Blue-DRAQ5 (nucleus); Scale bar−10 μm.