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. 2019 Apr 26;10:856. doi: 10.3389/fmicb.2019.00856

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Copyright © 2019 Caì, Yú, Jangra, Postnikova, Wada, Tesh, Whelan, Lauck, Wiley, Finch, Radoshitzky, O’Connor, Palacios, Chandran, Chiu and Kuhn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tibrovirus glycoproteins mediate virion entry into a broad range of animal cell types. Same experiment as in Figure 1B, 2 using different cell types exposed to rVSIV–VSIV G control and rVSIVs expressing diverse tibrovirus glycoproteins (G) (MOI = 3). (A) Bat (PESU-B5L, Ro5T, Ro6E, EidNi/41.3, EpoNi/22.1, RoNi/7.1, RoNi/7.2, HypNi/1.1, HypLu/45.1, Tb1 Lu, MyDauLu/47.1), nonhuman primate (Vero, MA104, RPGor53, S008397, RP00226), hispid cotton rat CRL, and boa constrictor JK cell lines. (B) Asian tiger mosquito C6/36 cells. The percentage of eGFP-expressing cell lines was measured by high-content imaging at 24 h post-exposure (bat, nonhuman primate, hispid cotton rat, and boa constrictor cell lines) or at 24, 48, 72, and 96 h post-exposure (Asian tiger mosquito cells). All experiments were performed in triplicate; error bars show standard deviations. BHV, Beatrice Hill virus; BASV, Bas-Congo virus; BAV, Bivens Arm virus; CPV, Coastal Plains virus; eGFP, enhanced green fluorescent protein; EKV-1, Ekpoma virus 1; EKV-2, Ekpoma virus 2; MOI, multiplicity of infection; SWBV, Sweetwater Branch virus; TIBV, Tibrogargan virus; rVSIV, recombinant vesicular stomatitis Indiana virus.