Table 1.

Strain and passaging history of the four authentic tibroviruses studied from the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) (The University of Texas Medical Branch (UTMB), 2018).

Tibrovirus	Passaging history prior to WRCEVA deposition
BAV strain UF-10/BT82-55	Isolated in 1982 from biting midges (Culicoides insignis) in Florida, United States, and then deposited to WRECA (Gibbs et al., 1989). Provided by WRECA for this study after 6 intracranial (IC) passages in suckling laboratory mice, 1 IC passage in a Syrian golden hamster (Mesocricetus auratus), and 1 passage in adult grivet (Chlorocebus aethiops) kidney Vero cells. Genomic sequence of this passaged virus reported in 2015 [GenBank #KM205019.1 (Walker et al., 2015) and GenBank # KP688373.1 (Lauck et al., 2015)]. Expanded for this study in Asian tiger mosquito (Aedes albopictus) C6/36 cells at 30°C until CPE affected approximately 60% of cells compared to non-infected control cells.
Coastal Plains virus (CPV) strain DPP 53	Isolated in 1981 at Coastal Plains Research Station, Northern Territory, Australia, from an apparently healthy steer (Cybinski and Gard, 1986). “Passaged in cell culture a number of times” (Gubala et al., 2011) [no further information available]. Genomic sequence of this passaged virus reported in 2011 [GenBank #GQ294473.1 (Gubala et al., 2011)]. Deposited at WRECA some time prior to 1995. Provided by WRECA for this study after 4 IC passages in suckling laboratory mice. Expanded for this study in C6/36 cells at 30°C until cytopathic effects (CPE) affected approximately 60% of cells compared to non-infected control cells.
SWBV strain UF-11	Isolated in 1981 from biting midges (C. insignis) in Florida, United States (Gibbs et al., 1989), and then deposited at WRECA. Provided by WRECA for this study after an unknown number of undescribed passages followed by 2 passages in C6/36 cells. Genomic sequence of this passaged virus reported in 2015 [GenBank #KM204997.1 (Walker et al., 2015)]. Expanded for this study in C6/36 cells at 30°C until CPE affected approximately 60% of cells compared to non-infected control cells.
TIBV strain UF-10	Isolated in 1976 from biting midges (Culicoides brevitaris) collected around Peachester, QLD, Australia, via inoculation of midge homogenates intracerebrally into suckling laboratory mice (1 passage) and then into BHK-21 cells (Cybinski et al., 1980). “Passaged in cell culture a number of times” (Gubala et al., 2011) [no further information available]. Genomic sequence of this passaged virus reported in 2011 [GenBank #GQ294472.1 (Gubala et al., 2011)]. Deposited at WRECA some time prior to 1995. Provided by WRECA for this study after 7 IC passages in suckling laboratory mice and 1 passage in Vero cells. Expanded for this study in Vero cells at 37°C until CPE affected approximately 60% of cells compared to non-infected control cells.