Table 3.
Microbiological testing and acceptance criteria for probiotic ingredients or finished products intended for oral use.
| Probiotic strain category | Test | Method | Acceptance criteria (CFU/g) |
|---|---|---|---|
| Contaminant microorganisms | |||
| Non-spore-forming | Non-lactic acid bacteria | ISO 13559/ IDF 153 | Not more than 5 × 103 |
| Total yeasts and molds | USP <2021> | Not more than 100 | |
| Spore-forming | Total yeasts and molds | Not more than 100 | |
| Yeasts and molds | Total aerobic microbial count | Not more than 1 × 103 | |
| Specified microorganisms | |||
| Non-spore-forming, spore-forming, yeasts and molds | Escherichia coli | USP <2022> | None detected in 10 g |
| Salmonella spp. | |||