Figure 6.

Lack of PD-1 signaling is associated with an increased frequency of CD103⁺ bT_RM (A) Number of D^bLT359 specific CD8 T cells in WT and PD-L1^−/− mice. (B) Frequency and number of brain CD103⁺ D^bLT359⁺ CD8 T cells in WT and PD-L1^−/− mice. (C) Frequency of CD103⁺D^bLT359⁺CD69⁺ CD8 T cells and viral DNA load at 30 dpi in brains of WT mice with control IgG or PD-L1 antibody delivered by i.c.v. cannulae. (D) gMFI of IRF4 expression by D^bLT359⁺ T cells at 9 and 45 dpi. (E) TCR affinities of D^bLT359 monomer-binding CD8 T cells determined by 2D-micropipette adhesion assay. Data are from a pool of 5 mice. (F) gMFI of PD-1, Tim3, and 2B4 (left panel), and Blimp-1, EOMES, and TCF-1 (right panel). (G) Frequency of TCF-1⁺CXCR5⁺ D^bLT359⁺ CD8 T cells from the brains of mice at 45 dpi. Data are from two to three independent experiments with 3–8 mice per group. Mann Whitney test between WT and PD-L1^−/− groups was performed in panels B, C, and D; two-way ANOVA with Tukey multiple comparison test in panel A was performed. Values represent mean ± SD; *p ≤ 0.05, **p ≤ 0.01, not significant (ns) p>0.05.