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. 2019 Apr 11;10:345. doi: 10.3389/fpls.2019.00345

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Copyright © 2019 Stai, Yadav, Sinou, Bruneau, Doyle, Fernández-Baca and Cannon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CYCLOIDEA gene tree, for species in subfamily Cercidoideae. For all species but Cercis (red), there are two gene copies: in the clades labeled “CYC1” (pink) and “CYC2” (orange). Where chromosome counts are available, the haploid count is indicated at the end of the sequence identifier. These values are 7 for the three included Cercis species, and 14 for all other species for which counts have been determined within the Cercidoideae, save Gigasiphon macrosiphon, which has 13. For C. canadensis, one sequence has been amplified using PCR and one sequence (Cerca190S17002) comes from the genomic assembly. One of several possible rootings is shown (with bootstrap support values indicated), based on comparison with CYCLOIDEA orthologs from Ceratonia oreothauma (carob relative, from the Caesalpinioideae) and Dussia macroprophyllata (an early-diverging species from the Papilionoideae).