Splitomicin-treated wild-type (wt) cells and sir2Δ
cells display similar transcriptional changes relative to untreated
wild-type cells. (A) Correlation of transcriptional
changes between genetic and chemical inactivation of Sir2p.
Transcriptional changes were determined by competitive hybridization to
DNA microarrays containing >6,000 yeast ORFs. The correlation plot
shows transcriptional changes in a sir2Δ mutant
relative to wild type (sir2Δ/wt) on the
vertical axis and changes in wild-type cells treated with splitomicin
relative to untreated wild-type cells (15 μM splitomicin/no
treatment) on the horizontal axis. (B) A Venn diagram
comparing genes up-regulated (Left) and down-regulated
(Right) more than 2-fold relative to wild-type or
untreated cells and sir2Δ, hst1Δ, or
splitomicin-treated wild-type cells. (C) Correlation of
transcriptional changes in wild-type cells in response to splitomicin
treatment with and without cycloheximide. The correlation plot shows
transcriptional changes in splitomicin- and cycloheximide-treated
wild-type cells relative to cells treated with cycloheximide alone (15
μM splitomicin CYH/CYH) on the vertical axis and changes in
wild-type cells treated with splitomicin relative to untreated
wild-type cells (15 μM splitomicin/no treatment) on the
horizontal axis. (D) Venn diagrams comparing
transcriptional changes (up- or down-regulation) in
hst2Δ, hst3Δ, and
hst4Δ cells and splitomicin-treated cells (split).