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. 2001 Dec 18;98(26):15113–15118. doi: 10.1073/pnas.261574398

Figure 2.

Figure 2

Splitomicin-treated wild-type (wt) cells and sir2Δ cells display similar transcriptional changes relative to untreated wild-type cells. (A) Correlation of transcriptional changes between genetic and chemical inactivation of Sir2p. Transcriptional changes were determined by competitive hybridization to DNA microarrays containing >6,000 yeast ORFs. The correlation plot shows transcriptional changes in a sir2Δ mutant relative to wild type (sir2Δ/wt) on the vertical axis and changes in wild-type cells treated with splitomicin relative to untreated wild-type cells (15 μM splitomicin/no treatment) on the horizontal axis. (B) A Venn diagram comparing genes up-regulated (Left) and down-regulated (Right) more than 2-fold relative to wild-type or untreated cells and sir2Δ, hst1Δ, or splitomicin-treated wild-type cells. (C) Correlation of transcriptional changes in wild-type cells in response to splitomicin treatment with and without cycloheximide. The correlation plot shows transcriptional changes in splitomicin- and cycloheximide-treated wild-type cells relative to cells treated with cycloheximide alone (15 μM splitomicin CYH/CYH) on the vertical axis and changes in wild-type cells treated with splitomicin relative to untreated wild-type cells (15 μM splitomicin/no treatment) on the horizontal axis. (D) Venn diagrams comparing transcriptional changes (up- or down-regulation) in hst2Δ, hst3Δ, and hst4Δ cells and splitomicin-treated cells (split).