Figure 2.

(A) Trehalose is hydrolyzed by porcine trehalase but compounds 2–4 are not. Compounds were incubated with trehalase for 1–8 hours, then reactions were analyzed by TLC. Lanes: (i) trehalose or analogue incubated 8 h without trehalase; (ii–v) trehalose or analogue incubated 1, 2, 4, or 8 h in the presence of trehalase; (vi) glucose (for 1) or corresponding glucose analogue (for 2–4) standards. (B) Compounds 2 and 4 inhibit porcine trehalase. Varying concentrations of analogues 2–4 were incubated in the presence of trehalose and trehalase, then released glucose was measured using a spectrophotometric assay and IC₅₀ values determined. No inhibition was observed for compound 3 using this assay. Error bars represent the standard deviation from three replicates.