Table 1.
Primer sequences for mRNA expression | ||
Genes | Forward primer (5’−3’) | Reward Primer (5’−3’) |
Akt | CCTCTGCTTTGTCATGGAGTAT | CACAATCTCCGCACCATAGAA |
Arc | CCTGAGCCACCTGGAAGAGTA | GGCCCATTCATGTGGTTCTG |
Mtor | GGTGTGGCATGTGGTTCTGT | CCATCCAATCTGATGCTGGA |
Per2 | TTGGTGTGTGGGTTGTTGTG | CTACCTGGTCAAGGTGCAAGAG |
PSD95 | TGACGACCCATCCATCTTTATC | CCCGGACATCCACTTCATT |
Spn | GATCCAAGTATTCAGCACCTACTC | CACTCGCTTCTCTAGCTCATATTC |
TrkB | ACTAAGATCCACGTCACCAATC | CAGGGTGTAGTCTCCGTTATTC |
b-Actin | TAAGGCCAACCGTGAAAAGATGAC | ACCGCTCGTTGCCAATAGTGATG |
Primer sequences for MeDIP and ChIP assays | ||
Genes | Forward primer (5’−3’) | Reward Primer (5’−3’) |
Akt | CTACTGGAGGGAGGTCTTCTAT | GTGATCTACCCATCTCTGGTTC |
Arc | AATAACCTGCCTTAGCCTCATC | CCGAGTGACTAATGTGCTCTG |
Mtor | CTCACGACTGATTGGCTCTT | GGGAGGAACATCCACCAATAA |
Per2 | GCTGGTCAGTTTAGGAAGTAGG | GGATGTCTCACACCGTCAATAA |
PSD95 | CATTGCCCTGAAGAACGC | ATGGATCTTGGCCTCGAA |
Spn | ATGAATCCAAGAAGGAGGACTTC | GATGAAGAAGACGAAGAGGACG |
TrkB | CAAAGACCCGTTACCGACTT | GTGATCTACCCATCTCTGGTTC |