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. Author manuscript; available in PMC: 2020 Mar 19.
Published in final edited form as: J Am Coll Cardiol. 2019 Mar 19;73(10):1173–1184. doi: 10.1016/j.jacc.2018.12.053

Figure 2. Deletion polymorphism results in increased cAMP-dependent promoter activity.

Figure 2.

(A) NRVMs were transfected with DEL or INS promoter constructs. 24 hours after transfection cells were treated with 1 µ mol/L 6-Benz-cAMP or 5 µmol/L enoximone for 48 hours. Higher cAMP levels specifically increased activity of the DEL promoter construct. (B) NRVMs were transfected with the DEL promoter construct +/− a CRE binding site. 24 hours after transfection cells were treated with cAMP for 48 hours. Deletion of the CRE site attenuated the response to cAMP. ANOVA/ Kruskal-Wallis’ test significance is shown in the Figure (*). N= 8 different transfections, 4–6 wells/transfection.