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. Author manuscript; available in PMC: 2020 Mar 19.
Published in final edited form as: J Am Coll Cardiol. 2019 Mar 19;73(10):1173–1184. doi: 10.1016/j.jacc.2018.12.053

Figure 3: ATF3 binds to the INS polymorphism and regulates PDE3A promoter activity.

Figure 3:

(A) EMSA of the 29 nucleotide probe and mutant. Lane 1: Wild type (WT) probe; 2: Wild type probe and HeLa nuclear extract; 3: Same as lane 2 plus ATF3 antibody; 4: Same as lane 2 plus YY1 antibody; 5: Same as lane 2 plus 100 ng of WT cold competitor; 6 Mutant (Mut) probe and HeLa nuclear extract. (B) EMSA of the 29 nucleotide probe and human left ventricle (LV) nuclear extracts. Lane 1: Wild type probe and human non-failing nuclear extract; 2: Same as Lane 1 plus ATF3 antibody; 3: Wild type probe and human non-failing nuclear extract; (C) NRVMs were transfected with luciferase constructs containing the INS, DEL, or mutant constructs. These results indicate ATF3 binds to the 29-nt INS.