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. 2019 Apr 18;15(4):e1007728. doi: 10.1371/journal.ppat.1007728

Fig 4. MKK2 participates in basal defense against virus.

Fig 4

(A) Location of single guide RNA target in NbSIPKK locus. An additional adenine was inserted into the exon of NbSIPKK, leading to the frame shift in the coding sequence. (B) nbsipkk-crispr plants displayed severe curl leaves with virus infection. Eight-leaf-period wild type and nbsipkk-crispr N. benthamiana leaves were infiltrated with A. tumefaciens harboring either TYLCCNV+TYLCCNB infectious clone, or empty vector. Plant phenotype was monitored 7 days after inoculation. (C) Western blot analysis of MAPK activation and virus titers of systemic leaves in wild-type or nbsipkk-crispr plants. Endogenous phosphorylated NbSIPK was monitored by immunoblot with an anti-pTEpY antibody. Virus amount was detected by the immunoblot assay with a TYLCCNV-CP antibody. (D) Relative accumulation levels of TYLCCNV in agroinfiltrated wild type and nbsipkk-crispr plants. Viral accumulation was determined by qPCR at 7 dpi. The values represent viral DNA accumulation relative to levels in TYLCCNV infected wild type plants. The data are shown as means and standard error of the mean (SEM) of three biological replicates. Different letters indicate significant differences among samples (p<0.05, Student’s t test).