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. 2019 May 3;14(5):e0215604. doi: 10.1371/journal.pone.0215604

Fig 5. Reverse transcription–polymerase chain reaction (RT-PCR) and Western blot analysis in rabbit vessels.

Fig 5

(A) RT-PCR expression of TNF-α, RAGE, HMG CoA reductase, iNOS, AGTR1, and Arg-1. (B-G) Comparisons of relative mRNA expression, normalized to expression of GAPDH as the housekeeping gene. (H) Western blot expression of TNF-α, RAGE, iNOS, and Arg-1. (I-L) Representative data showing protein expression, normalized to expression of GAPDH as the housekeeping gene. Data in the bar graphs are quantified ratios of the signal for TNF-α, RAGE, HMG CoA reductase, AGTR1, iNOS, and Arg-1 relative to the signal for GAPDH, presented as fold increases. Data are mean±SEM. * p<0.05 vs. positive control group, † p<0.05 vs. statin group, and ‡ p<0.05 vs. ARB group. AGTR1, angiotensin II receptor type 1; ARB, angiotensin II receptor blocker; Arg-1, arginase-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HMG CoA, 5-hydroxy-3-methylglutaryl–coenzyme A; iNOS, inducible nitric oxide synthase; RAGE, receptor for advanced glycation end products; TNF-α, tumor necrosis factor-α.