Table 3. Target site and sequences of shRNA.
pGPU6/GFP/Neo-HMGB1-homo-539 | |
Target sequence 1 | GCGAAGAAACTGGGAGAGATG |
Sense strand 5′-3′ | GCGAAGAAACTGGGAGAGATGTTCAAGAGACATCTCTCCCAGTTTCTTCGCTTTTTTG |
Antisense strand 3′-5′ | CGCTTCTTTGACCCTCTCTACAAGTTCTCTGTAGAGAGGGTCAAAGAAGCGAAAAAAC |
pGPU6/GFP/Neo-shNC | |
Target sequence 1 | GTTCTCCGAACGTGTCACGT |
Sense strand 5′-3′ | GTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTTG |
Antisense strand 3′-5′ | CAAGAGGCTTGCACAGTGCAAAGTTCTCTTGCACTGTGCAAGCCTCTTAAAAAAC |