Figure 10.
The ncu06238 gene is not required for spore killing. (A) Asci from a cross between two SkS strains (ISU-4559 × P8-43), where one of the strains has had its ncu06238 coding sequence deleted. While some normal asci are detected, many asci are abnormal and some mimic the Spore killer phenotype (red arrows). (B) Asci from a cross between an SkS strain and an Sk-2 strain (ISU-4559 × ISU-4561), where both strains have had their ncu06238 coding sequence deleted. Nearly all viable progeny isolated from this cross have the Sk-2 genotype (34 out of 35; Figure S2).