Figure 11.

Replacement of AH36³²¹¹ with AH36^Sk-2 restores spore killing to an rfk-1 mutant. (A) Strain ISU-4562 was constructed by replacing AH36³²¹¹ in ISU-3222 (upper diagram, red box) with nat1 (using vector v160, with deleted positions indicated with an orange bar; ISU-3222 is a descendant of ISU-3211). Strain ISU-4563 was then constructed by replacing AH36^Δ::nat1 in ISU-4562 with AH36^Sk-2::hph (lower diagram, red box and green rectangle). The locations of the two recombination flanks used to replace AH36^Δ::nat1 with AH36^Sk-2::hph are indicated with black crosses and red dotted lines. (B–D) Asci are from crosses between F2-23 and (B) ISU-3222, (C) ISU-4562, and (D) ISU-4563. (E–G) Asci are from crosses between ISU-4348 and (E) ISU-3222, (F) ISU-4562, and (G) ISU-4563. See Figure 9 for a description of the v150^Δ::hph allele. B, black; hph, hygromycin resistance cassette; W, white.