Figure 12.

rfk-1 is transcribed in cultures undergoing sexual reproduction. (A) Sequences of Sk^S × Sk-2 poly(A)-enriched RNA (top, FGSC 1767 × FGSC 7426) and Sk^S × Sk^S poly(A)-enriched RNA (bottom, FGSC 1767 × FGSC 1766) were aligned to AH36⁷⁴²⁶. The y-axis values indicate the number of times each position within AH36⁷⁴²⁶ is found in an aligned RNA sequence. The x-axis values indicate the position of each base within AH36⁷⁴²⁶ with respect to chromosome III of N. intermedia FGSC 7426. These data suggest that rfk-1 is transcribed in Sk^S × Sk-2 perithecial cultures and that rfk-1 RNA is specific to Sk^S × Sk-2 crosses. A model of rfk-1 gene structure is shown above the chart. (B) Sequences of Sk^S × Sk-2 (top, FGSC 1767 × FGSC 7426) and Sk^S × Sk^S (bottom, FGSC 1767 × FGSC 1766) poly(A)-enriched RNA were aligned to actin. In contrast to the AH36⁷⁴²⁶ alignment data, RNA sequences from both Sk^S × Sk-2 and Sk^S × Sk^S crosses similarly align to actin with respect to number and pattern. The x-axis values are for the actin locus on chromosome V of FGSC 1766.