Table 1. List of mutations the fixed during the initial growth adaptation of ID8 and NC28.

Lineage	Protein function	Protein name	Aa position	$\text{Δ}\mathrm{Aa}$	Nuc. position	$\text{Δ}\mathrm{Nuc}$
ID8	DNA packaging	C	80	N $\to$ S	1958	A $\to$ G
	Coat	F	355	P $\to$ S	3628	C $\to$ T
	Spike	G	171	T $\to$ A	4493	A $\to$ G
	DNA pilot	H	82	T $\to$ A	4770	C $\to$ T
	DNA pilot	H	135	H $\to$ Y	4930	G $\to$ A
NC28	Scaffolding (lysis)	D (E)	129 (69)	A (K) $\to$ S (N)	2903	G $\to$ T
	Coat	F	272	silent	3904	A $\to$ G
	DNA pilot	H	10	V $\to$ A	5116	T $\to$ C

A list of all mutations that fixed during the initial growth optimization of ID8 and NC28 performed by Rokyta et al. (2009), including the nucleotide and amino acid changes, and a description of the affected proteins. Certain regions of the bacteriophage genome have overlapping genes, indicated with the second affected gene in parentheses. Aa, amino acid; Nuc., nucleotide.