Figure 2.

Analysis of RNA-Seq data reveals highly consistent gene up- and downregulation. (A) Plots comparing RNA-Seq replicates within a genotype demonstrate extremely high concordance. Pearson’s r correlation values are shown. (B and C) Browser shots of RNA-Seq signals (normalized read depth) at the sas-4 and asl loci for the three indicated genotypes. y-axis values (shown for asl) are the same for each genotype. (D) MA plots reveal genes significantly up- (red dots below midline) or downregulated (red dots above midline), in comparisons of sas-4 to WT (left) or asl to WT (right). (E and F) Venn diagrams demonstrating the highly significant overlap of genes found to be up- (E) or downregulated (F) in both sas-4 and asl, relative to WT. RNA-Seq, RNA-sequencing; WT, wild-type.