Table 5. Candidate genes screened for interaction with sas-4.

RNAi gene target	Stock identified	Crossed to MS>GFP alone	Crossed to MS>GFP;sas-4 RNAi
Upd2	BDSC 33949	Strongly dysmorphic	Strongly dysmorphic
	BDSC 33988	Not determined	Normal
Ets21C	BDSC 39069	Strongly dysmorphic	Strongly dysmorphic
Castor	VDRC 2929	Normal	Normal
Cnc	VDRC 37674	Normal	Normal
	VDRC 108127	Normal	Normal
	VDRC 101235	Strongly dysmorphic	Strongly dysmorphic
Dif	VDRC 30579	Normal	Normal
	VDRC 100537	Normal	Normal
G6PD	VDRC 101507	All erect, 5% blistered and 7% furled	All erect, 29% blistered and 11% furled
	BDSC 50667	Strongly dysmorphic	Strongly dysmorphic
ImpL3	VDRC 110190	Normal	Normal
	VDRC 31192	Normal	Normal
Jra	VDRC 31595	Normal	Necrotic spots
KEAP1	VDRC 107052	Normal	Normal
	VDRC 330323	Normal	Normal
TRAF4	VDRC 110766	Normal	Normal
MMP1	VDRC 31989	Normal	Normal
WWOX	VDRC 108350	Normal	Normal

Based on cellular function, a subset of genes was selected from the list of genes that were upregulated in both sas-4 and asl mutants relative to wild-type. The stock identifiers for RNAi lines targeting the genes of interest are shown. RNAi lines were crossed to the MS1096-Gal4 UAS-GFP (MS>GFP) wing disc driver alone and in combination with sas-4 RNAi. Glucose-6-phosphate dehydrogenase knockdown alone had modest effects that were synergistically elevated in cells codepleted of centrosomes by sas-4 RNAi (see Figure 6, A and B). RNAi, RNA interference; BDSC, Bloomington Drosophila Stock Center; VDRC, Vienna Drosophila Resource Center.