Table 2. Plasmids used in this study.
Plasmid | Source/reference |
---|---|
p3XFLAG:KanMX | Gelbart et al. (2001) |
pGAC24 | Lesser and Guthrie (1993) |
pGAC24-CYC1 terminator | Steinmetz and Brow (2003) |
pGAC24-YOP1 3′UTR | This study |
pGAC24-YOP1 3′UTR-mut1 | This study |
pGAC24-YOP1 3′UTR-mut1+mut2 | This study |
Plasmids were used for endogenous, 3XFLAG tagging or as the termination reporter (Figure 6).