Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Species (Drosophila melanogaster) | 26F05-LexA | BDSC | 54702 | Expressed in A08a neurons |
| Species (D. melanogaster) | 26F05-Gal4 | BDSC | 49192 | Expressed in A08a neurons |
| Species (D. melanogaster) | 165-Gal4 | W. Grueber | N/A | Expressed in dbd neurons |
| Species (D. melanogaster) | UAS-LacZ | BDSC | 8529 | Control transgene |
| Species (D. melanogaster) | UAS-LacZ | BDSC | 8530 | Control transgene |
| Species (D. melanogaster) | UAS-unc-5::HA | B. Dickson | N/A | UAS drives unc-5 |
| Species (D. melanogaster) | UAS-robo-2::HA | BDSC | 66886 | UAS drives robo-2 |
| Species (D. melanogaster) | UAS-bruchpilot(short)-mstrawberry | S. Sigrist | N/A | UAS drives fluorescently labeled truncated bruchpilot |
| Species (D. melanogaster) |
10xUAS-IVS-myr::smGdP::HA, 13xLexAop2-IVS-myr::smGdP::V5 | BDSC | 64092 | UAS drives HA membrane tag, LexAop drives V5 membrane tag |
| Species (D. melanogaster) | UAS-MCFO | BDSC | 64090 | UAS drives multi-colored-flip-out |
| Species (D. melanogaster) | UAS-DenMark, UAS-syt.eGFP | BDSC | 33064 | UAS drives DenMark, UAS drives synaptotagmin::GFP |
| Species (D. melanogaster) | 13XLexAop2-IVS-p10-GCaMP6m, 20xUAS-CsChrimson-mCherry | V. Jayaraman | N/A | LexAop drives GCamp6m, UAS drives Chrimson |
| Antibody, monoclonal | Mouse anti-V5 | Invitrogen, Carlsbad, CA, | Cat. R96025, Lot 1949337 | (1:1000) |
| Antibody, polyclonal | Rabbit anti-mCherry | Novus Biologicals, Littleton, CO | Cat. NBP2-25157, Lot 102816 | (1:500) |
| Antibody, monoclonal | Rat anti-HA | Roche Holding, AG, Basel, Switzerland | Cat. 11867423001, Lot 27573500 | (1:100, after suggested dilution) |
| Antibody, monoclonal | Rat anti-OLLASDyLight-650conjugated antibody | Novus Biologicals, Littleton, CO | Cat. NBP1-06713C, Lot F-090517c |
(1:100) |
| Antibody, polyclonal | Chicken anti GFP | Aves Labs, Inc, Tigard, OR | Cat. GFP-1020, Lot. GFP697986 |
(1:1000) |
| Antibody, polyclonal | Rabbit anti-mCherry | Novus Biologicals, Littleton, CO | Cat. NBP2-25157, Lot 102816 | (1:500) |
| Antibody, secondary | Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H + L) | Jackson ImmunoResearch, West Grove, PA | Cat. 715-545-151 | (1:400) |
| Antibody, secondary | Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (H + L) | Jackson ImmunoResearch, West Grove, PA | Cat. 711-295-152 | (1:400) |
| Antibody, secondary | Alexa Fluor 647 AffiniPure Donkey Anti-Rat IgG (H + L) | Jackson ImmunoResearch, West Grove, PA |
Cat. 712-605-153 | (1:400) |
| Antibody, secondary | Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H + L) | Jackson ImmunoResearch, West Grove, PA |
Cat. 703-545-155 | (1:400) |