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. 2019 Apr 24;8:e45252. doi: 10.7554/eLife.45252

Figure 2. F. tularensis enters the endocytic pathway in recipient cells after cell-cell transfer.

Figure 2.

(A) Representative image of F. tularensis (green) inside an EEA-1 (red) positive vacuole 10 min after synchronized cell-cell transfer. (B) Representative image of F. tularensis (green) inside a LAMP-1 (red) positive vacuole 1 hr after synchronized cell-cell transfer. (C–D) The percentage of cells with at least one bacterium enclosed inside of (C) EEA-1 or (D) LAMP-1 positive vacuoles. The black line represents a conventional infection where the BMDMs phagocytose extracellular bacteria. The blue line represents purified recipient BMDMs after bacterial transfer. From three independent experiments with 50 infected cells counted per experiment per time point. Student t-test. Mean ± standard deviation. *p<0.05, **p<0.01, ***p<0.001. ns- no statistical significance. No time points were significantly different for LAMP-1 co-localization.