Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | EEA-1 (goat monoclonal) | Santa Cruz Biosciences | clone N19, catalog number sc-6415 | 1:50 dilution, permeabilized in 0.1% saponin, 2% FBS in humidifier at 37 degrees Celcius for 30 minutes. Cells cannot be fixed for longer than 7 minutes prior to staining. |
| Antibody | LAMP-1 (rat monoclonal) | Developmental Studies Hybridoma Bank | Clone 1D4B | 1:500 dilution after stock mixed with 50% glycerol, permeabilized in 0.1% saponin, 2% FBS in humidifier at 37 degrees Celcius for 30 minutes |
| Antibody | Anti-rat secondary (goat monoclonal) |
ThermoFisher Scientific | catalog number 26-4826-82 | 1:500 dilution in0.1% saponin, 2% FBS in humidifier at 37 degrees Celcius |
| Antibody | Anti-goat secondary (donkey monoclonal) | ThermoFisher Scientific | catalog number PA1-28662 | 1:500 dilution in 0.1% saponin, 2% FBS in humidifier at 37 degrees Celcius |
| Strain, strain background (Mus musculus, female) | 6–10 week old female C57Bl/6J | Jackson Labs | catalog number 000664 | |
| Strain, strain background (Francisella tularensis, Live Vaccine Strain) | F. tularensis or LVS | CDC | ||
| Strain, strain background (Francisella tularensis, Schu S4) | F. tularensis Schu S4 | BEI Resources | ||
| Strain, strain background (Francisella tularensis, inducibleT6SS mutant) | T6SS mutant | this paper |
F. tularensis strain with an in-frame, markerless deletion of FTL_0119 (dotU) that is complemented with a pEDL17 plasmid containing dotU under the tetracycline inducible promoter and GFP on a constitutive promoter |
|
| Strain, strain background (Francisella tularensis, Empty Vector Control) |
WT | this paper | wildtype LVS that is complemented with a pEDL17 plasmid containing a kanamycin resistance cassette under the tetracycline inducible promoter and GFP on a constitutive promoter |
|
| Strain, strain background (Francisella
tularensis, Empty Vector in ΔdotU) |
ΔdotU | this paper | A FTL_0119 deletion in LVS that is complemented with a pEDL17 plasmid containing a kanamycin resistance cassette under the tetracycline inducible promoter and GFP on a constitutive promoter | |
| Chemical compound, drug |
anhydrous tetracycline | Cayman Chemicals | catalog number100009542 |