Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug |
fluorescently-labeled RNA probes for VGAT, VGLUT, VACHT |
Molecular instruments | ||
| Chemical compound, drug |
SlowFade Gold | Invitrogen | ||
| Chemical compound, drug |
perampanel | Santa Cruz Biotech; Adooq Bioscience |
||
| Chemical compound, drug |
picrotoxin | Tocris | ||
| Equipment | 700 nm and 505 nm led light sources |
Mightex | ||
| Equipment | light meter | Extech Instruments | ||
| Recombinant DNA reagent |
Ciona robusta
pVGAT > CFP |
Yasunori Sasakura, University of Tsukuba, Japan |
||
| Recombinant DNA reagent |
C. robusta opsin promoter (as pSP-Ci-opsin1) |
Takeo Horie, Tsukuba University, Japan; Takehiro Kusakabe, Konan University, Japan |
||
| Recombinant DNA reagent |
synthesized RFP | Gene Block; IDT | ||
| Recombinant DNA reagent |
C. robusta
pAMPAR > GFP |
Haruo Okado, Tokyo Metropolitan Institute of Medical Science, Japan |
See Hirai et al., (Hirai et al., 2017). |
|
| Recombinant DNA reagent |
C. robusta
pOpsin > RFP |
This paper. | This construct was created using the opsin promoter (Horie and Kusakabe) and a synthesized RFP (Gene Block, IDT). |
|
| Recombinant DNA reagent |
C. robusta
pVGAT > H2B::RFP |
This paper. | This construct was created using the Gateway cloning system, cloning pVGAT into pDONR-221-P3-P5, then recombining with an H2B::RFP entry clone. |
|
| Software, algorithm |
Python v 2.7 | Python Software Foundation |
||
| Software, algorithm |
Microsoft Excel | Microsoft | ||
| Software, algorithm |
Imaris v 9.1 | Bitplane | ||
| Software, algorithm |
MATLAB | WolframAlpha | ||
| Software, algorithm |
ELIANE script for MATLAB |
This paper. | Estimator of Locomotion Iterations for Animal Experiments; a MATLAB script for tracking swimming larvae during behavioral assays. |
|
| Strain, strain background |
C. intestinalis | Marine Biological Laboratory, Woods Hole, Massachusetts, USA |
Formerly known as C. intestinalis type B. |
|
| Strain, strain background |
C. robusta | Santa Barbara Harbor, USA; S. Lepage, M-REP, Carlsbad, USA |
Formerly known as C. intestinalis type A. |
|
| Strain, strain background |
pVGAT > kaede, C. robusta stable line |
National Bioresource Project, Japan |
||