Figure 3. Axl knockdown of tumor cells decreases endothelial cell migration and invasion.

Endothelial cells were seeded in the transwell migration or invasion chambers in the presence of EGM-2-MV medium or the tumor cell conditioned medium. The number of migrated (A) or invaded (B) endothelial cells were counted 24 h later. Tumor cells and the endothelial cells were co-cultured. Tumor cells were seeded on the bottom of the transwell chambers, and endothelial cells were seeded on the transwell chamber insert. Endothelial cells were allowed to migrate or invade for 24 h, and the number of migrated (C) or invaded (D) endothelial cells were counted. Results are the mean and standard error values of three (n = 3) independent experiments. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001; by two-way ANOVA.