Figure 4. Axl knockdown of tumor cells suppresses tumor cell-induced angiogenesis in vivo.

shScramble or Axl knockdown tumor cells were inoculated intradermally at four sites on the ventral surface of athymic nu/nu mice (10⁵ cells per nodule). (A) Representative images of MDA-MB-231 tumor nodules. The number of vessels recruited per tumor nodule was counted 3 days later for MDA-MB-231 (B) and DU-145 (C) cells. Results are the mean and standard error values, with twelve nodules per group analyzed. ^****p < 0.00001; by one-way ANOVA.