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. 2019 May 3;9:6871. doi: 10.1038/s41598-019-42862-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The microbial community was observed to remain relatively constant over the study period as measured by Observed OTUs (A), Shannon-Wiener (B) and Simpson diversity (C), and Bray-Curtis dissimilarity (D). Samples were categorized based on the time interval relative to first sample as: 0 (n = 29), 0–2 years (n = 36), 2–4 (n = 22), and 4 + (n = 46) years since initial sample collection. The median and interquartile ranges (IQR) are represented by the middle, top, and bottom lines of each boxplot. No statistically significant comparisons were observed.