Fig. 1.

Quiescent EM and CM T-cells are metabolically active. a Glycolytic stress profile of NV, EM and CM T-cells by measuring ECAR before and following injections of oligomycin (0.75 μM), FCCP (1 μM) and antimycin A and rotenone (1 μM) at the time points indicated. Basal (b) and maximal ECAR (c) in NV, EM and CM T-cells. d Representative immunoblots from two different donors per cell type for GLUT1, HKI HKII, GAPDH, PFKP, PKM2 and LDHA and β-actin. Respective densitometry normalised to β-actin is shown. Data are either representative of five independent experiments (a–c) or 3−4 experiments (d). Statistical analysis was performed using a non-matching one-way ANOVA with Tukey’s multiple comparison test (b–d). For non-parametric data, a Kruskal−Wallis test with Dunn’s multiple comparisons test was used. Data expressed as mean ± SEM; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001