Fig. 7.

Disrupted glutaminolysis causes compensatory TCA cycling. a NV T-cells were activated (anti-CD3/CD28) in the presence or absence of STAT5i (100 μM) for 4 h. Relative abundance of glucose-derived ¹²C and ¹³C TCA cycle metabolites citrate, α-ketoglutarate, succinate, fumarate and malate. b Mass isotopologue distributions (MID) of TCA cycle intermediates citrate, α-ketoglutarate, succinate, fumarate and malate. c Relative abundance of glucose-derived ¹²C and ¹³C amino acids aspartate and proline. Data are representative of four experiments and are expressed as mean + SEM