Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 3;9:6895. doi: 10.1038/s41598-019-43319-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Considerable variation in K_d values among the epitope tag antibody clones. (A) (Left panel) Binding curves of the tested antibody clones against the monomeric form of the epitope tags. The antibody concentrations used for IP were as follows: 0.2 nM for anti-FLAG (M2, IE6, FLA-1 and L5) and anti-V5 (V5-10 and 6F5); 0.1 nM for anti-HA (3F10 and 4B2) and anti-PA (NZ-1); and 0.05 nM for anti-Ty1 (BB2). (Right panel) Error curves for the best-fitting K_d. In each plot, the obtained apparent K_d value in nM is shown with the 95% confidence interval. (B) Affinity comparison of the antibody clones shown in panel A. Error bars depict the plus and minus confidence interval of the K_d value.