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. 2019 May 3;9:6895. doi: 10.1038/s41598-019-43319-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of tag multimerisation on the apparent affinity of the antibodies. (Left panel) The binding curves of anti-epitope tag antibody clones against monomeric, dimeric and/or trimeric forms of the epitope tags shown Figs 2 and 4 are simultaneously plotted for comparison purposes. (Right panel) Affinity comparison. The error bars depict the 95% confidence intervals for the K_d values.