. 2019 May 3;9:6895. doi: 10.1038/s41598-019-43319-y

Table 2.

Detailed overview of characterised antibodies.

Antibody type	Supplier	Clone	Species	IgG subclass	Bead type^a	Tag	K_d (nM)	95% Confidence interval of K_d (nM)
Anti-FLAG	Sigma	M2	Mouse	IgG1	α-mouse IgG beads	FLAG	0.76^b	0.44–1.3
	Sigma	M2	Mouse	IgG1	α-mouse IgG beads	FLAGx3	0.21	0.12–0.37
	Wako	IE6	Mouse	IgG2b	α-mouse IgG beads	FLAG	1.8	1.3–2.6
	Wako	IE6	Mouse	IgG2b	α-mouse IgG beads	FLAGx3	0.50	0.40–0.62
	MBL	FLA-1	Mouse	IgG2a κ	α-mouse IgG beads	FLAG	1.3	0.82–2.0
	MBL	FLA-1	Mouse	IgG2a κ	α-mouse IgG beads	FLAGx3	0.33	0.22–0.50
	BioLegend	L5	Rat	IgG2a	α-rat IgG beads	FLAG	0.44	0.27–0.72
	BioLegend	L5	Rat	IgG2a	α-rat IgG beads	FLAGx3	0.16	0.097–0.20
Anti-HA	Roche	3F10	Rat	IgG1	α-rat IgG beads	HA	0.38	0.22–0.70
	Roche	3F10	Rat	IgG1	α-rat IgG beads	HAx3	0.067	0.035–0.12
	Wako	4B2	Mouse	IgG2b	α-mouse IgG beads	HA	6.6^b	5.5–8.0
	Wako	4B2	Mouse	IgG2b	α-mouse IgG beads	HAx3	0.88	0.50–1.5
Anti-V5	Sigma	V5-10	Mouse	IgG1	α-mouse IgG beads	V5	0.59	0.37–0.95
						V5x2	0.36	0.22–0.58
						V5x3	0.23	0.15–0.35
	Wako	6F5	Mouse	IgG2b	α-mouse IgG beads	V5	0.42	0.22–0.77
						V5x2	0.28	0.16–0.47
						V5x3	0.28	0.18–0.44
Anti-PA	Wako	NZ-1	Rat	IgG2a	α-rat IgG beads	PA	0.65^b	0.38–1.1
						PAx2	0.38	0.26–0.54
						PAx3	0.27	0.17–0.42
Anti-Ty1	Sigma	BB2	Mouse	IgG1	α-mouse IgG beads	Ty1	0.39^b	0.22–0.65
						Ty1x2	0.29	0.17–0.48
						Ty1x3	0.20	0.12–0.34

^aAnti-IgG magnetic beads used to capture the antibodies.

^bK_d values obtained by combining data from two independent experiments.