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. 2019 May 3;10:2045. doi: 10.1038/s41467-019-10025-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Knockdown of lnc-TALC restores TMZ sensitivity in TMZ-resistant GBM cells in vitro. a Determination of TMZ IC50 by CCK-8 assay analysis after knockdown of the top 10 upregulated lncRNAs in 229R and HG7R cells upon TMZ treatment (100 μM) at the indicated concentrations for 72 h (n = 3). b Association heatmap between lnc-TALC expression and functional gene sets. c CCK-8 assay analysis revealed the effect of lnc-TALC knockdown on the TMZ-resistant cells with TMZ treatment at the indicated concentrations for 72 h (n = 3). d Flow cytometric analysis revealing the effect of lnc-TALC knockdown on the apoptosis of TMZ-resistant cells with TMZ treatment (100 μM) for 72 h (n = 3). e Colony formation assay detecting the effect of lnc-TALC knockdown on the growth of TMZ-resistant cells with TMZ treatment in a 6-well dish (300 cells per well) for 11 days (n = 3). Representative images and the average number of colonies are shown. f EdU assay showing the effect of lnc-TALC knockdown on DNA replication activity of TMZ-resistant cells with TMZ treatment (100 μM) for 72 h (n = 3). g Western blot analysis of indicated proteins in TMZ-resistant and parental GBM cells transfected with LV-CRISPR-lnc-TALC, LV-lnc-TALC or scramble. Data are presented as the mean ± S.D. P value was determined by Student’s t-test or one-way ANOVA. Significant results were presented as NS non-significant, *P < 0.05, **P < 0.01, or ***P < 0.001