Fig. 4.

Met regulated by lnc-TALC is highly expressed in recurrent GBM and is required for TMZ resistance. a The levels of RTK molecules were analyzed in mRNA microarray profiles. b Western blot analysis of p-Met and c-Met levels in TMZ-resistant and parental GBM cells. c qRT-PCR analysis of the effect of lnc-TALC knockdown on MET expression in TMZ-resistant GBM cells. d Western blot analysis of c-Met and p-Met levels affected by lnc-TALC knockdown in TMZ-resistant GBM cells. e qRT-PCR analysis of the effect of lnc-TALC overexpression on MET expression in parental GBM cells. f Western blot analysis of c-Met and p-Met levels affected by lnc-TALC overexpression in parental GBM cells. g Colony formation assay of the effect of MET overexpression on 229R knock down of lnc-TALC with TMZ treatment (100 μM) in a six-well dish (300 cells per well) for 11 days (n = 3). The average number of colonies is shown. h Western blot analysis of the indicated proteins in 229R GBM cells after lnc-TALC knockdown or MET overexpression. i Colony formation assay of the effect of sh-MET on LN229 overexpressing lnc-TALC with TMZ treatment (100 μM) in a 6-well dish (300 cells per well) for 11 days (n = 3). The average number of colonies is shown. j Western blot analysis of the indicated proteins in LN229 GBM cells after lnc-TALC overexpression or MET knockdown. k Colony formation assay of the effect of SGX-523(200 nM) on LN229 cells overexpressing lnc-TALC with TMZ treatment (100 μM) in a six-well dish (300 cells per well) for 11 days (n = 3). The average number of colonies is shown. l Western blot analysis of the indicated proteins in LN229 GBM cells after SGX-523 (200 nM) treatment. Data are presented as the mean ± S.D. P value was determined by Student’s t-test. Significant results were presented as NS non-significant, *P < 0.05, **P < 0.01, or ***P < 0.001