Fig. 6.

The Lnc-TALC/c-Met axis activating the Stat3/p300 complex increases MGMT expression by modulating the acetylation of histone H3. a Western blot analysis of MGMT expression in TMZ-resistant and parental GBM cells. b Western blot analysis of MGMT expression in TMZ-resistant GBM cells transfected with LV-CRISPR-lnc-TALC. c Immunofluorescence analysis of MGMT and c-Met in TMZ-resistant GBM cells transfected with sh-MET. The nuclei were stained with DAPI. Scale bar = 10 μm. d ChIP assay of the enrichment of p300 in the MGMT promoter region normalized to IgG in LN229 and 229R GBM cells (n = 3). e Left: Immunoprecipitation of 229R and LN229 GBM cells with an antibody against p-Stat3 followed by labeling with an anti-p300 antibody. Right: Immunoprecipitation of 229R and LN229 GBM cells with an antibody against p300; the cells were subsequently labeled with the anti-p-Stat3 antibody. f ChIP assay of the enrichment of H3K9ac in the MGMT promoter region normalized to IgG in LN229 and 229R GBM cells (n = 3). g ChIP assay of the enrichment of p300 in the MGMT promoter region normalized to IgG in 229R GBM cells transfected with LV-CRISPR-lnc-TALC and si-Stat3 (n = 3). h Left: Immunoprecipitation of 229R GBM cells after knocking down lnc-TALC with an antibody against p-Stat3 followed by labeling with an anti-p300 antibody. Right: Immunoprecipitation of 229R GBM cells knocking down lnc-TALC with an antibody against p300 followed by labeling with an anti-p-Stat3 antibody. i ChIP assay of the enrichment of H3K9ac in the MGMT promoter region normalized to IgG in 229R GBM cells transfected with LV-CRISPR-lnc-TALC and si-Stat3 (n = 3). Data are presented as the mean ± S.D. P value was determined by Student’s t-test. Significant results were presented as NS non-significant, *P < 0.05, **P < 0.01, or ***P < 0.001