Fig. 7.

EIF4A3 and stress granule formation. a The effect of T-595 and T-598 on stress granule marker accumulation. HeLa cells were treated with 10 μM compounds (T-598, or T-595) for the indicated time a alone or b with sodium asernite (Ars, 0.1 mM) added either for the last 1 h or c. after the first hour and then incubated for the remainder of the experiment then lysed and probed with the respective antibodies as indicated, by Western Blotting. Stress granule quantification and visualization in HeLa cells with the indicated markers (DAPI for DNA, G3BP and TIA-1 for stress granules (SGs)) on treatment with 10 μM compounds (T-595 or T-595) or DMSO (UT) as control, with 0.5 mM sodium arsenite added d 1 h prior to harvest or e treated only for the first hour and then incubated without for 24 h. d Average number of SGs per cell at 4 h or e the total number of cells displaying ≥3 SGs at 24 h were reported or f representative images captured on a confocal microscope at the indicated times. Scale bar = 10 μm