Comparison of FcγRIIIa binding and antibody-mediated effector functions of BMS-986012 and fucosylated parental antibody 7E4. Data are representative ofat least three independent experiments. A, FcγRIIIa receptor binding. BMS-986012demonstrates enhanced binding to CHOcells stablyexpressing human FcγRIIIa with binding EC5qs of 3.5 and 138 nmol/L for BMS-986012 () and 7E4 (), respectively. B, Enhanced BMS-986012-mediated FcγRIIIa-dependent ADCC activity in vitro. NK effector cells and DMS79 target cells were incubated with either BMS-986012 () or 7E4 (). Median percent-specific lysis ± SD is plotted against antibody concentration. C, BMS-986012-mediated ADCP activity in vitro. Macrophage effector cells and DMS79 target cells were incubated with BMS-986012 (), 7E4 (), or isotype control antibodies IgG1nf () and IgG1 (). Macrophage-phagocytosed tumor cells were identified by flow cytometry as PKH26+ CD14+ double-positive events. The median percent of double-positive events ± SD is plotted against antibody concentration. D, BMS-986012-mediated CDC activity in vitro. DMS79 target cells were incubated with BMS-986012 (), 7E4 (), or isotype control antibodies IgG1nf () and IgG1 () plus human complement. Cell viability was determined using CellTiter-Glo, and the percentage of viable cells is plotted against Ab concentration.