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. 2019 May 4;52:27. doi: 10.1186/s40659-019-0235-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Western blot analyzed TBX4 protein level in the hindgut/rectum of the normal (N) and ARM (A) groups. TBX4 was detected as an approximately 35-kDa (kd) band on Western blots (a). Immunoblots showed a strong signal for TBX4 protein in the normal group, but a weak signal in the ARM group. Histone-H3 protein was used as an internal control. A histogram shows the trends of TBX4 expression at each time point (b). A peak can be noted on E15. Values are presented as mean ± SD. *Significant difference from the normal group