Fig. 2.
Cell viability of explants. The viability of cells at the cartilage interfacial region at the vicinity of the chondral incision in osteochondral explants was viewed by live-dead staining on day 3 and day 21 of culture. Explants (n = 3 per group) were incubated in 6 μM calceinAM and 4 μM ethidium homodimer-1 solution for 1 h at 37 °C under aseptic conditions. Confocal micrographs viewed from the top or superficial zone of cartilage depict live (green) cells and dead (red) cells at 10× magnification. White arrowheads indicate the leading edge of the chondral incision. Scale bar represents 200 μm