Fig. 5.

Safranin O and collagen II staining of osteochondral explants. Incised osteochondral explants cultured for 28 days under cLIUS at 14 kPa (5 MHz, 2.5 Vpp), 20 min, 4 times/day in the presence or absence of IL-6 or TNFα (n = 6 explants per group). Non-cLIUS-stimulated explants served as controls (n = 6 explants per group). Explants fixed in 10% neutral buffered formalin were paraffin embedded. The panel shows 4 μm sections of osteochondral explants at the interfacial region stained with safranin O (a-f) and collagen II (g-l) at 20× magnification. Scale bar represents 400 μm. Insets depict the whole section imaged at 2× magnification. Black arrows in the insets depict the sites of incision