Fig. 6.

CB₂ knockdown promoted HCC cell mobility and activated AKT signaling pathway NC: HCC cells were transfected with siNC (50nM); CB₂-KD: HCC cells were transfected with CB₂ siRNA (50nM); MDA19 + CB₂-KD: HCC cells were transfected with CB2 siRNA (50nM) and treated with MDA19 (30μM for Hep3B and 40μM for HepG2) for 48 h. a Cell migration and (b) cell invasion were detected by transwell assay. c AKT signaling pathway components, including AKT, p-AKT, CDK4, CDK6 and Cyclin D1, were detected by western blot and analyzed by Image J software. All experiments were performed at 3 times. *P < 0.05