Fig. 11.

Influence of NH₄Cl on ZIKV adhesion to the host cells. Flow cytometry analysis of viral adhesion to cells in the presence of NH₄Cl was carried out. Vero cells pre-treated with 50 mM NH₄Cl were overlaid with ZIKV stock and following 2 h incubation at 4 °C, they were fixed and ZIKV was immunostained with Alexa Fluor 488. Graph shows median fluorescence normalized to control, which corresponds to the proportion of ZIKV-positive cells in total cells population. vØ – inhibitor-untreated cells overlaid with ZIKV; NH₄Cl –NH₄Cl-treated cells overlaid with ZIKV; mock – mock-overlaid inhibitor-untreated cells. The data is presented as mean ± SD. To determine the significance of differences between compared groups, single-factor analysis of variance (ANOVA) was applied. P values < 0.05 were considered significant. One asterisk (*) identifies adjusted P values between 0.01 and 0.05, two asterisks (**) identify adjusted P values between 0.01 and 0.001, three asterisks (***) identify adjusted P values between 0.001 and 0.0001