Fig. 14.

NH₄Cl interfere with late stages of ZIKV infection. Vero cells were treated Baf A1 and NH₄Cl in 3 settings: (pre + vir) prior to + during ZIKV infection (t = 1 h + 2 h); (all) prior to, during and post-ZIKV infection (t = 1 h + 2 h + 72 h) or (post) post-ZIKV infection only (t = 72 h, starting from the time point 2 h p.i.). 3 days p.i. viral RNA was isolated from medium and quantified with RT-qPCR. mock – mock infected cells; vØ – ZIKV- infected cells; NH₄Cl – 50 mM NH₄Cl; BafA1–100 nM bafilomycin A1; control – inhibitor-untreated, non-infected cells. To determine the significance of differences between compared groups, single-factor analysis of variance (ANOVA) was applied. P values < 0.05 were considered significant. One asterisk (*) identifies adjusted P values between 0.01 and 0.05, two asterisks (**) identify adjusted P values between 0.01 and 0.001, three asterisks (***) identify adjusted P values between 0.001 and 0.0001