Fig. 3.

Inhibition of ZIKV infection in Vero cells by chemical agents blocking clathrin-mediated endocytosis. Vero cells pre-treated with CME inhibitors were infected with ZIKV and viral yield was assessed 3 days p.i. Virus yield (RT-qPCR) is presented on the left side of the graph, while on the right side toxicity of the compounds is visualized (XTT assay). mock – mock-infected cells; vØ – ZIKV-infected cells; AMTD – 400 μM amantadine, Dyn – 100 μM dynasore, MM – 20 μM MitMab, PtS – 50 μM PitStop; control – inhibitor-untreated, non-infected cells. The data is presented as mean ± SD. To determine the significance of differences between compared groups, single-factor analysis of variance (ANOVA) was applied. P values < 0.05 were considered significant. One asterisk (*) identifies adjusted P values between 0.01 and 0.05, two asterisks (**) identify adjusted P values between 0.01 and 0.001, three asterisks (***) identify adjusted P values between 0.001 and 0.0001