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. 2019 May 3;12:42. doi: 10.1186/s13041-019-0463-2

Table 1.

Primers used in real-time PCR analyses

Target gene Primers sequences Size (bp)
β-Actin Forward 5′-GGGAAATCGTGCGTGAC-3′ 176
Reverse 5′-AGGCTGGAAAAGAGCCT-3′
IL-1β Forward 5′-ATGTGCTGCTGCGAGATTTGA-3′ 136
Reverse 5′-TGCCACCTTTTGACAGTGATG-3′
IL-6 Forward 5′-TTGGGAGTGGTATCCTCTGTGAA-3′ 88
Reverse 5′-CCTTCTTGGGACTGATGCTGG-3′
TNF-α Forward 5′-CACCCCGAAGTTCAGTAGACA-3′ 184
Reverse 5′-TCCCTCCAGAAAAGACACCAT-3’
Bax Forward 5′-AGGATGCGTCCACCAAGA-3’ 194
Reverse 5′-AAAGTAGAAGAGGGCAACCAC-3’
Bcl-2 Forward 5′-CGGGAGAACAGGGTATGA-3’ 149
Reverse 5′-CAGGCTGGAAGGAGAAGAT-3’
NLRP3 Forward 5′-ATGTTGCCTGTTCTTCCA-3’ 130
Reverse 5′-GGGTCTCCCAGAGTATTG-3’
ASC Forward 5′-AGCAAGAGTAAAAGGTGACCG-3’ 103
Reverse 5′-TTGAGTTCATCCCCTGACAAG-3’
Nrf2 Forward 5′-CAGTGCTCCTATGCGTGAA-3’ 109
Reverse 5′-GCGGCTTGAATGTTTGTC-3’
HO-1 Forward 5′-ACAGAGGAACACAAAGACCAG-3’ 136
Reverse 5′-GTGTCTGGGATGAGCTAGTG-3’
NQO1 Forward 5′-TACGATCCTCCCTCAACA-3’ 108
Reverse 5′-TACAGCAGCCTCCTTCAT-3’